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Routine Sequencing


AGRF's Routine Sequencing Services are NATA accredited and comprise of three different services:


Routine Sequencing Service Process


Seq Purified DNA

Purified DNA (PD) Service

AGRF's purified DNA service is based on submission of purified DNA template (plasmid or PCR product) with the appropriate primer. Please refer to Sample Submission Requirements for additional details.

The purified DNA sequencing service provides:

 

For each sample, our Bioinformatics service provides a suite of analysis files (see below), available in electronic format via our website.  The turnaround time for this service is between 3 - 5 days from receipt of sample and the appropriate primer.

Unpurified PCR Product (PD+) Service

AGRF's PD+ service is designed for users requiring high-volume purification and sequencing of PCR amplicons. Please refer to Sample Submission Requirements for additional details.

The PD+ sequencing service provides:

 

For each sample, our Bioinformatics service provides a suite of analysis files (see below), available in electronic format via our website.  The average turnaround time for this service is 10 - 12 days from receipt of sample and the appropriate primer.

Capillary Separation (CS) Service

AGRF's capillary separation service is for clients who have completed the BDT sequencing reaction and subsequent cleanup themselves. Samples are sent in dried down pellet format in tubes or plates and are analysed on the AB 3730xl sequencing platforms. The average turn around time for this service is 2 working days from sample receipt. 

The capillary separation service process flow is outlined above.  For each sample, our Bioinformatics service provides the client with a suite of analysis files which are available in electronic format via our secure website. 


Sample Submission Requirements including details regarding accepted templates and chemistries are linked here.


ABI Prism BigDye Terminator Sequencing Reaction Kits:
BDT order form and Purchase orders can be faxed to us on (07) 3365 1823.

 

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