Plasmid Clone Sequencing
Using the AB 3730xl Platform
AGRF offers a NATA accredited Plasmid / Clone Sequencing service, whereby EST/SAGE libraries that are provided by the client are routinely sequenced. SAGE are derived from mRNA and contain short sequence tags indicative of the number of times that a specific mRNA sequence is observed in a given tissue/cell type. ESTs represent portions of expressed genes and are derived from partial or whole segments of cDNA, which are cloned into bacteria to generate EST libraries. Common applications using SAGE include the comparison of gene expression in tumor versus normal tissue and also expression patterns in tissues/cells at different developmental stages, whilst ESTs are used to identify gene transcripts from the tissue/cell type of interest leading to gene discovery through sequence determination.
AGRF provides several sequencing options for EST/SAGE libraries, whereby Applied Biosystems BDTv3.1 chemistry can be used with either standard M13 standard sequencing primers, or with custom primers supplied by the client. Dual strand sequencing is also available for an additional charge. Plasmid / Clone sequencing is a large scale service with automated pipelines offered for a minimum of 384 samples/clones. The AGRF Bioinformatics section offer EST assembly support.
Plasmid / Clone Sequencing: Sample Submission Requirements
Please refer to the Plasmid / Clone sequencing Service Process flow diagram outlined below for details regarding the components of this service.
Plasmid / Clone Sequencing Service Process
