Platforms
AGRF's Sequencing service has the capability and experience to process sequencing projects of any scale. A suite of automated liquid handlers, thermal cyclers, AB 3730xl capillary sequencers, a GS FLX sequencer and an illumina GA II (short read sequencer), place AGRF as the leading provider of sequencing services. The AGRF Sequencing Service is NATA accredited with a custom built LIMS sample tracking system that provides the means to track sample progress through our pipeline.
AB 3730xl
AGRF operates the AB3730xl 96-capillary sequencer for low to high throughput DNA sequencing. Its automated features, such as an integrated autosampler, plate stacker and internal bar code reader enhance the AB 3730xl run capacity. Advanced polymers combined with superior optical sensitivity, enable this platform to produce uniform signal profiles and long read lengths. AGRF's Bioinformatics team utilises leading sequence analysis software enabling high quality base calling and flexibility to accommodate a variety of applications.
Using the AB 3730xl platform AGRF provides the following Sequencing Services:
- Sequencing – Capillary Separation
- Sequencing – Purified DNA
- PCR Re-Sequencing
- Plasmid / Clone Sequencing
- Shotgun Sequencing
Using the AB 3730xl platform AGRF also provides the following service:
AGRF also utilises the AB3730 platform. AGRF provides the following services using this platform:
Roche GS FLX
AGRF utilises the Roche GS FLX sequencing platform, providing unparalleled read capacity with state-of-the-art sequencing technology. Many applications have been developed for this instrument, including the large scale analysis of bacterial/yeast genomes, BAC pools, transcriptomes, microRNA libraries and ultra deep Amplicon sequencing. In addition, the new application of paired-end sequencing enables contig alignment, greatly reducing the time for bacterial/yeast genome finishing. The GS FLX is capable of producing up to 400,000 reads in a single day with an average read length of over 200 bp or 80 Mb total sequence at 99.5% raw base accuracy. The AGRF's Bioinformatics team utilises leading sequence analysis software enabling high quality base calling and flexibility to accommodate a variety of applications.
Using the Roche GS FLX platform AGRF provides the following Sequencing Services:
Regarding the following applications, please contact us:
- Methylation Analysis
