We are excited to invite you to join us for an exclusive free online event to hear about Australia's first PacBio Sequel II service, delivering even more highly accurate long reads to accelerate your science.
We've put together a great panel of speakers, from The University of Queensland, AGRF and PacBio to take you through their experiences with the PacBio systems, with the new HiFi reads, and they will be able to answer any questions you might have about the services.
Speaker information is listed below, but you can download the full agenda using the button below.
We look forward to "seeing" you there :)
Speaker Information
Dr David Hawkes - PacBio Service Manager, AGRF
David Hawkes is the Brisbane Site manager at AGRF, where he manages services in Sanger sequencing, MassARRAY genotyping and PacBio long read sequencing. He completed his PhD at the University of Queensland where he worked towards harnessing cytochrome P450’s for environmental remediation, and later rounded out his training with a Masters in Biostatistics. Pursuing an interest in genomics, David worked with Keith Mitchelson, on a novel sequencing by assisted mutagenesis (SAM) technique for Sanger sequencing, before taking a position with AGRF in the MassARRAY genotyping team. Working as genotyping manager and later Brisbane node manager, David has 14 years of experience working within genomics services. David has previously managed AGRF’s HiSeq4000 service and now managers PacBio services on the Sequel II platform.
Dr CX Chan - Senior Research Fellow Australian Centre for Ecogenomics, University of Queensland
Dr Chan is a Senior Research Fellow at the Australian Centre for Ecogenomics, at UQ's School of Chemistry and Molecular Biosciences. He has a PhD in Genomics and Computational Biology from UQ. His team is using advanced computational and database approaches to study genome evolution of microbes, including bacteria, algae and protists, and to develop highly scalable phylogenomic approaches. His research routinely involves de novo assembly and analysis of high-throughput sequencing data.
Angelika Christ - Facility Manager of the IMB Sequencing Facility, University of Queensland
Angelika Christ leads a team of sequencing specialists, producing high-quality sequencing libraries and next-generation sequencing data. She and her team help find researchers the best approach to their sequencing projects — ranging from agriculture, to drug development and medical research — and ensure the best outcomes. Angelika has 15 years of commercial and research laboratory experience. She has worked in the field of next-generation sequencing since 2010 on various technology platforms and applications. Angelika holds a Dipl.-Ing (FH) in Biochemistry from the Mannheim University of Applied Sciences in Germany and a brings experience in organic chemistry and peptide chemistry as well as cell culture and assay development to her work at the IMB Sequencing Facility.
Dr Khi Pin, Chua - Scientist, Field Applications Support, Bioinformatics PacBio Asia Pacific
Khi Pin completed his post-graduate degree in computational study of proteins. In his post-doctoral research, he integrated genomics and transcriptomics data to elucidate insights into the evolution and resistance mechanism of lung cancer. Khi Pin is currently working for PacBio to support customers utilizing the cutting-edge PacBio long-reads sequencing.
Ana Vuckovic - Business Development Officer – Research Infrastructure
The University of Queensland is a research intensive university and houses a large suite of capabilities, instrumentation and services which support all researchers – ranging from basic and life sciences, human biomedical sciences, through to plant, animal, and environmental sciences, and including the physical sciences. Ana represents the UQ based facilities which offer a range of services, including imaging, characterisation, genomics, molecular engineering, recombinant protein capabilities, nanotechnology, and many more to support researchers and industry nationwide.
Abstracts
Applications of Long and Accurate Hi-Fi Sequencing with PacBio Sequel II, by Dr Chua Khi Pin
The release of the PacBio Sequel II System in 2019 brought dramatic throughput improvements and protocols for producing a new data type, highly accurate long reads or HiFi reads. PacBio is the only sequencing technology to offer highly accurate long reads (HiFi reads) that provide Sanger-quality accuracy (>99%). HiFi reads allow a new sequencing paradigm whereby there is no longer a need to choose between accuracy or read length. This has greatly advanced many sequencing applications including de-novo assembly of genomes, full-length transcriptomics characterization and metagenomics study of complex population. From the bioinformatics aspect, HiFi reads are smaller, faster and simpler to analyze. In this talk, I will introduce the characteristics of PacBio HiFi reads and its advantages in downstream analysis of various applications.
Are dinoflagellate genomes the final frontier? by Dr CX Chan
Genomes of dinoflagellate algae are well-known to be highly idiosyncratic, with gene features atypical in eukaryotes. In this presentation, I will talk about our experience with PacBio in generating de novo genome data and full-length transcriptome data from these algae, focusing on the symbiont species in corals and coral reefs.
