Your Partners in Next Generation Sequencing

We offer a full range of Next Generation Sequencing services, including library preparation, sequencing and bioinformatics analysis.

Our services are run by experienced staff who are easily contactable via phone or email. We are available to give advice at any stage of your project and we can assist you in finding a suitable sequencing solution.

We are proud to be Australia’s most comprehensive supplier of NextGen services, offering a range of speeds and outputs.

RNA-Seq

RNA sequencing (RNA-Seq) is powerful method for qualitative and quantitative analysis of the transcriptome of any organism. RNA-Seq is used for differential gene expression, detection of alternate splicing and characterisation of novel transcripts.  

  • mRNA-Seq : targeting poly(A) RNA provides a sensitive option for lowly expressed coding transcripts
  • Whole Transcriptome : RNA sample are enriched for both mRNA and non-coding RNAs to provide a comprehensive view of the transcriptome
  • Small RNA : targets small regulatory RNAs such as miRNA for profiling and discovery.

    • Whole Exome Sequencing

    Whole exome sequencing (WES) is a powerful tool for investigation of genetic variance across the protein coding regions of the genome. WES provides high coverage and high confidence in germline variant calls for the study of rare mendelian disorders, complex disease and cancer. AGRF provides comprehensive solutions for WES, including bioinformatics using gold standard tools (Illumina DRAGEN).

  • Twist Exome v2 : Discover the power of the Twist Exome 2.0 panel (36.5 Mbp) designed for detecting rare disease, inherited conditions as well as germline cancers. Delivering high-quality data with superior coverage based on major genetic databases (RefSeq, CCDS, GenCode, ClinVar, ACMG73), addressing inherited diseases including cystic fibrosis (CFTR) and targeting other clinically relevant markers.1 Additionally, you have the option to include the mtDNA genome as a spike-in probe set upon request, or you can introduce your Twist compatible probe spike-in for a customised service. Additional charges may apply.

  • Twist Alliance Clinical Research Exome : Our comprehensive NGS service includes the Broad Institute designed Twist Alliance Clinical Research Exome panel (34.9 Mbp). This panel covers an extensive range of clinically relevant areas of the genome linked to cancers, rare diseases and inherited conditions. This panel is built on the Twist Core Exome backbone, providing enhanced coverage with the inclusion of the mtDNA genome at no extra cost, along with boosted coverage for Online Mendelian Inheritance in Man (OMIM), Catalogue of Somatic Mutations in Cancer (COSMIC) and non-exonic regions such as ACMG73.2

    • Whole Genome Sequencing

    Whole genome sequencing (WGS) provides the most comprehensive analysis of genome variance and structure. WGS enables accurate detection of SNV, and structural variation of human genomes, or de novo assembly and variant detection of non-human genomes. 

    AGRF offers a range of services for sequencing of small microbial genomes, large complex genomes, as well as a clinical genome sequencing service for rare mendelian disorders, complex disease and cancer.

  • Human WGS : accurate SNV detection using the Illumina NovaSeq platform with or without variant calls. For clinical samples we offer NATA-accredited (ISO15189 Medical Testing) workflow.
  • Small WGS : AGRF offers a sample preparation and sequencing options suitable for a single or hundreds of microbial genomes.
  • Low Pass WGS : accurately measure genome-wide variance in populations. Applications range from GWAS to trait-mapping and selection in agriculture.

    1. Exome 2.0 - Twist BioScience, accessed 15 October 2023,
      Twist BioScience Exome 2.0
    2. Alliance Panels - Twist BioSicence, accessed 15 October 2023,
      Twist Bioscience Alliance Panels

     

    Our NGS Services

    • RNA-Seq

    • Whole Genome Sequencing 

    • Whole Exome Sequencing 

    • Target Sequencing 

    • Amplicon Sequencing 

    • Metagenomics

    • MethylSeq

    • Whole Plasmid Sequencing

     
     

    ILLUMINA NOVASEQ X PLUS

    Illumina NextSeq 2000

    illumina nExtseq 2000

     

    Advance your genomic research.

    Your Partners in Next Generation Sequencing.